Properties of Hydrangea Ringspot Virus Ribonucleic Acid

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Abstract
Hydrangea ringspot virus [from Hydrangea macrophylla] was purified to near homogeneity. Virus nucleic acid was identified as single-stranded RNA with a sedimentation value of 33.6 S before and 21.1 S after formaldehyde denaturation. Nucleic acid MW determinations made by linear log sucrose density gradient centrifugation gave values of 2.25 .times. 106 and 2.14 .times. 106, respectively, for native and formaldehyde-treated RNA. Electrophoresis on polyacrylamide gels before and after formaldehyde denaturation gave MW values of 2.64 .times. 106 and 2.03 .times. 106. The values from formaldehyde denatured RNA are considered the most reliable. Thermal denaturation profiles showed a Tm [melting point] of 55.2.degree. C in 0.1 M-sodium phosphate, pH 7.0 and a hyperchromicity of 22.4%.