Histamine Content and Histidine Decarboxylase Activity in the Spleen of the Magnesium-Deficient Rat: Comparison with the Skin and Peritoneal Mast Cells

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Abstract
In young rats, some effects of Mg depletion in the diet on histamine content and histidine decarboxylase (HDC) activity in spleen were studied in comparison with those in the skin and peritoneal mast cells. In young rats fed a Mg-deficient diet (0.001% Mg), the splenic histamine contents increased to levels about 1.3, 2.8 and 23 times as high as those in rats fed a control diet (0.07% Mg) on the 4th, 6th and 8th day, respectively; histamine contents in the peritoneal mast cells also increased to about 1.2 and 1.8 times in the control levels on the 6th and 8th day, respectively; no change was observed in histamine contents in the skin during 8 days of Mg depletion. HDC activities in the spleen of Mg-deficient rats on the 4th, 6th and 8th day increased to levels about 5.5, 15.5 and 35 times as high as the respective control values; the activities in the skin increased to about 37, 7 and 10 times the control values on the 4th, 6th and 8th day, respectively; while in the peritoneal mast cells, the activities increased to about 1.2 and 2.2 times the control values on the 6th and 8th day, respectively. On the 8th day of Mg deficiency, some studies were made on the effects of histamine releasers on histamine contents in the spleen and peritoneal mast cells. Compound 48/80 (0.5 .mu.g/ml) or polymyxin B (5 .mu.g/ml) induced a histamine release from the peritoneal mast cells, but not from the spleen cells isolated from the Mg-deficient rat in vitro. The effects of Mg deficiency on the spleen cells and on the mast cells were evidently different.