An Electron Microscope Study of Basophile Substances of Frozen-Dried Rat Liver
Open Access
- 25 May 1958
- journal article
- research article
- Published by Rockefeller University Press in The Journal of cell biology
- Vol. 4 (3) , 291-300
- https://doi.org/10.1083/jcb.4.3.291
Abstract
Small pieces of liver from rats subjected to different dietary regimes were fixed by freeze-drying, and postfixed by in vacuo heating and denaturation with alcohol. Specimens were digested with ribo- or deoxyribonuclease, and stained with gallocyanin-chromalum, azure II, the Feulgen procedure or alcoholic platinic tetrabromide. Some specimens were reserved as controls of the effects of enzyme treatment. Stained and unstained specimens were embedded in methacrylate and examined by light and electron microscopy.Keywords
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