RAPID ANALYSIS AND PURIFICATION OF POLYMERASE CHAIN-REACTION PRODUCTS BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY

  • 1 May 1990
    • journal article
    • research article
    • Vol. 8  (5) , 546-+
Abstract
This report describes the use of high-performance liquid chromatography (HPLC) for the rapid analysis and purification of the polymerase chain reaction products. Employing a new anion-exchanges nonporous column, efficient separations of both DNA restriction fragments and amplified PCR products are achieved in 10 to 20 minutes and quantitated within .+-. 10%. The performance of the HPLC technique is described in terms of resolution, reproducibility, sensitivity and micropreparative capability and compared to that of gel electrophoresis for this application.

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