ANALYSIS OF COMMERCIAL POLLEN EXTRACTS BY ENZYME DETERMINATION .1. COMPARISON OF RAST-INHIBITION ASSAY AND ENZYME TITRATION FOR ORCHARD GRASS, RYE GRASS AND SHORT RAGWEED POLLEN EXTRACTS

  • 1 January 1980
    • journal article
    • research article
    • Vol. 45  (5) , 310-315
Abstract
Allergenic potency assessed by the 50% RAST[radioallergosorbent test]-inhibition endpoint titration was compared with enzyme titration for the analysis of commerical pollen extracts. Six different extracts of short ragweed, orchard grass and perennial rye grass were examined. A good correlation (P < 0.01) was found between the acid phosphatase titers and allergenic potency of short ragweed and perennial rye grass extracts. A similar correlation (P < 0.01) was observed between leucine aminopeptidase titers and allergenic potency of short ragweed extracts. No correlation could be found with orchard grass extracts. Enzymatic activity may provide a method for the control of certain commercial pollen extracts [used for human hyposensitization therapy].

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