Role of 2-ketobutyrate as an alarmone in E. coli K12: Inhibition of adenylate cyclase activity mediated by the phosphoenolpyruvate: Glycose phosphotransferase transport system
- 1 March 1984
- journal article
- research article
- Published by Springer Nature in Molecular Genetics and Genomics
- Vol. 193 (3) , 467-472
- https://doi.org/10.1007/bf00382085
Abstract
2-ketobutyrate and its analogues were found to inhibit strongly and transiently the rate of β-galactosidase synthesis in Escherichia coli K12. This effect was ascribed to a strong and transient inhibition of the adenylate cyclase activity. By using pts mutants, we showed, in agreement with our previous results (Daniel et al. 1983), that the likely target of 2-ketobutyrate and its analogues is the phosphoenolpyruvate: glycose phosphotransferase transport system (PTS). Furthermore, evidence for such a cascade effect caused by 2-ketobutyrate and its analogues allowed us to corroborate our previous proposal (Daniel et al. 1983) that 2-ketobutyrate, a precursor of isoleucine, acts as an E. coli alarmone monitoring the passage from anaerobic to aerobic growth conditions.This publication has 21 references indexed in Scilit:
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