Evidence against protein-induced ‘internal pressure’ in biological membranes. Partition of 8-anilinonaphthalene-1-sulphonate into Triton X-100 micelles and submitochondrial particles

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Abstract
It has recently been proposed that although small amphiphilic molecules partition into phospholipid vesicles this partition is reduced by a factor of 103–104-fold by the presence of proteins in biological membranes [Conrad & Singer (1979) Proc. Natl. Acad. Sci. U.S.A. 76, 5202–5206 and (1981) Biochemistry 20, 808–818]. However, the affinity with which 8-anilinonaphthalene-1-sulphonate partitions into, or binds to, Triton X-100 micelles and submitochondrial particles is very similar and therefore does not support this proposal.