A Reverse Genetics Approach for Recovery of Recombinant Influenza B Viruses Entirely from cDNA
Open Access
- 15 November 2002
- journal article
- research article
- Published by American Society for Microbiology in Journal of Virology
- Vol. 76 (22) , 11744-11747
- https://doi.org/10.1128/jvi.76.22.11744-11747.2002
Abstract
The recovery of recombinant influenza A virus entirely from cDNA was recently described (9, 19). We adapted the technique for engineering influenza B virus and generated a mutant bearing an amino acid change E116G in the viral neuraminidase which was resistant in vitro to the neuraminidase inhibitor zanamivir. The method also facilitates rapid isolation of single-gene reassortants suitable as vaccine seeds and will aid further investigations of unique features of influenza B virus.Keywords
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