TRANSFER OF RADIOACTIVE HEME AND FORMATION OF HEMOGLOBIN
- 25 February 1960
- journal article
- letter
- Published by Oxford University Press (OUP) in The Journal of Biochemistry
- Vol. 47 (2) , 269-271
- https://doi.org/10.1093/oxfordjournals.jbchem.a127062
Abstract
Upon incubating (pH 7.4,37[degree]C,4 hr.) a mixture of the supernatant of hemolyzate, protoporphyrin, Fe59Cl3, and Fe-incorporating factor (the cholate extract of duck erythrocyte stroma) in the presence of cysteine, Fe59-heme-containing hemoglobin (Hb) was synthesized. Synthesized Hb is entirely dependent on the supernatant of hemolyzates of the respective species, as characterized by Amber-lite IRC 50 column chromatography. A heme-acceptor of a globin nature contained in the supernatant is probably participating.This publication has 2 references indexed in Scilit:
- THE EFFECTS OF INHIBITORS ON Fe59-INCORPORATION INTO HEME BY DUCK HEMOLYSATEThe Journal of Biochemistry, 1958
- BIOSYNTHESIS OF HEME AND HEMEPROTEINS IN TISSUE CELLSThe Journal of Biochemistry, 1958