Production of Sepiapterin in Escherichia coli by Coexpression of Cyanobacterial GTP Cyclohydrolase I and Human 6-Pyruvoyltetrahydropterin Synthase
Open Access
- 1 June 2002
- journal article
- Published by American Society for Microbiology in Applied and Environmental Microbiology
- Vol. 68 (6) , 3138-3140
- https://doi.org/10.1128/aem.68.6.3138-3140.2002
Abstract
Synechocystis sp. strain PCC 6803 GTP cyclohydrolase I and human 6-pyruvoyltetrahydropterin synthase were coexpressed in Escherichia coli . The E. coli transformant produced sepiapterin, which was identified by high-performance liquid chromatography and enzymatically converted to dihydrobiopterin by sepiapterin reductase. Aldose reductase, another indispensable enzyme for sepiapterin production, may be endogenous in E. coli .Keywords
This publication has 18 references indexed in Scilit:
- Tetrahydrobiopterin: a critical cofactor for eNOS and a strategy in the treatment of endothelial dysfunction?American Journal of Physiology-Heart and Circulatory Physiology, 2001
- l-Ascorbic Acid Potentiates Endothelial Nitric Oxide Synthesis via a Chemical Stabilization of TetrahydrobiopterinJournal of Biological Chemistry, 2001
- Endothelial Dysfunction of Coronary Resistance Arteries Is Improved by Tetrahydrobiopterin in AtherosclerosisCirculation, 2000
- Tetrahydrobiopterin biosynthesis, regeneration and functionsBiochemical Journal, 2000
- Identification of the genes encoding enzymes involved in the early biosynthetic pathway of pteridines inSynechocystissp. PCC 6803FEMS Microbiology Letters, 1999
- The Complete Genome Sequence of Escherichia coli K-12Science, 1997
- Structural and Functional Consequences of Mutations in 6-Pyruvoyltetrahydropterin Synthase Causing Hyperphenylalaninemia in HumansPublished by Elsevier ,1995
- Human carbonyl and aldose reductases: New catalytic functions in tetrahydrobiopterin biosynthesisBiochemical and Biophysical Research Communications, 1991
- Human liver 6-pyruvoyl tetrahydropterin reductase is biochemically and immunologically indistinguishable from aldose reductaseBiochemical and Biophysical Research Communications, 1989
- BIOSYNTHESIS AND METABOLISM OF TETRAHYDROBIOPTERIN AND MOLYBDOPTERINAnnual Review of Biochemistry, 1985