Cytochemical detection of hyaluronidase activity in single human and mouse sperm by an improved substrate-film technique.
Open Access
- 1 January 1984
- journal article
- research article
- Published by SAGE Publications in Journal of Histochemistry & Cytochemistry
- Vol. 32 (1) , 63-66
- https://doi.org/10.1177/32.1.6690600
Abstract
A substrate-film method is described that allows the detection of hyaluronidase activity in nearly 100% of single human and mouse sperm. The level of hyaluronidase activity as determined by halo diameters was greater in mouse than in human sperm. This simple method may have use as a screening method for identifying compounds that cause developmental or genetic defects in male germ cells, or for the diagnosis of infertility due to decreased hyaluronidase activity.This publication has 0 references indexed in Scilit: