Identification of a Cell-Surface Protein Involved in the Binding Site of Sindbis Virus on Human Lymphoblastoic Cell Lines Using a Heterobifunctional Cross-Linker

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Abstract
A cell-surface protein of MW 90,000 was identified as the major protein located at or near the binding site of Sindbis virus on the JY and Daudi [human] lymphoblastoid cell lines. This Sindbis-virus-binding protein was detected by cross-linking Sindbis virus, modified with 4-[6-formyl-3-azidophenoxy]butyrimidate, to the cell surface. The applicability of the compound in the identification of specific cell-surface proteins was demonstrated using a monoclonal antibody reactive with .beta.-2-microglobulin, a protein which is associated with HLA-A, HLA-B and HLA-C.