Evaluation of a Colorimetric Test for the Determination of α-Amylase with p-Nitrophenylheptaoside as Substrate

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Abstract
A continuous method for the determination of .alpha.-amylase activity with a defined substrate was published by Rauscher et al. (1981). It offers the following advantages: good practicability, easy adaptation to analyzers and short incubation time. Laboratories (21) took part in a multicentric study on this method, working with different instruments and manual procedures at 3 temperatures. Additionally, all laboratories participated in a laboratory improvement program, in order to discriminate systematic from accidental errors. Data on imprecision, linearity, interferences and comparability of the method were presented at a workshop. The resulting coefficients of variation for imprecision in series as well as from day to day were 0.6-6.3% and 1.3-7.7%, respectively. No interferences from bilirubin, glucose, maltose and 37 selected drugs were found. The results correlate well with those from an UV-test (substrate: maltoheptaose). Compared with other methods, the expected systematic deviations were observed. Reference values for 25.degree., 30.degree. and 37.degree. C were indicated. [Human serum and urine samples were studied.].

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