Comparison of antiglobulin, direct, and anticomplement immunofluorescent staining for the identification of cytomegalovirus in cultured human fibroblasts

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Abstract
The sequential development of intracellular antigens in cultured human embryonic fibroblasts infected with the AD-169 strain of cytomegalovirus was studied by means of antiglobulin, direct, and anticomplement immunofluorescent staining.Direct immunofluorescent staining, although less sensitive than the antiglobulin or anticomplement methods for the detection of early diffuse nuclear antigens, gave the clearest definition of the typical intracellular inclusions produced by cytomegalovirus.Quantitative complement fixation assays, carried out in parallel with anticomplement staining, showed that complement is fixed strongly by the intracellular antigens which develop in the late stages of infection.

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