Secretin/Cholecystokinin-Stimulated Secretion of Trypsinogen and Trypsin Inhibitor in Pure Human Pancreatic Juice Collected by Endoscopic Retrograde Catheterization

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Abstract
Pure pancreatic juice was collected by endoscopic retrograde catheterization of the papilla of Vater from 10 fasting patients without known pancreatic disease. Secretin was given i.v. as a bolus dose at the beginning of the examination and cholecystokinin was given in the same way 15 min later. The juice was siphoned for 25 min and collected in 1 min fractions. Pancreatic secretory trypsin inhibitor [PSTI], and trypsinogen were measured by an immunochemical method. Secretin produced an increase in H2CO3 and a decrease in Cl secretion. Cholecystokinin caused a prompt increase in the concentration of both PSTI and trypsinogen. The ratio between these was constant, suggesting a strictly parallel secretion. The effect of cholecystokinin on secretion of total protein when given as bolus dose was of short duration, with a half-life of action of about 3.5 min.