ANTIBODY PRODUCTION STUDIED BY MEANS OF LOCALIZED HAEMOLYSIS IN GEL (LHG) ASSAY .2. ASSAY PROCEDURE

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  • 1 January 1968
    • journal article
    • research article
    • Vol. 14  (1) , 69-+
Abstract
The localized hemolysis in gel (LHG or plaque) assay was investigated and it was shown that the best condition for the assay is a plate made up from a standard tissue culture medium (199) in an agarose gel. Several convenient but sub-optimal alternatives are described. Other variables that were investigated were the concentration of indicator erythrocytes in the assay plate, the amount of complement added, the incubation time and the neutralization of xenogeneic developing serum by free mouse serum gamma-globulins. The reasons for using log (x +1) transformed data for statistical analysis are also presented.