Interaction Between Iodothyronines and Thyrotropin Receptor in Human Cultured Thyroid Cells
- 1 January 1981
- journal article
- research article
- Published by Taylor & Francis in Journal of Receptor Research
- Vol. 2 (4) , 367-382
- https://doi.org/10.3109/107998981809038873
Abstract
In order to verify the existence of a “short-loop” negative feedback between iodothyronines and adenylate cyclase system of human thyroid, we have studied the effect of preincubation with iodothyronines, iodotyrosines, iodothyronine analogues and iodide on TSH-induced cAMP cellular accumulation in normal human thyroid cells in primary culture. Iodide did not produce an inhibitory effect on TSH-dependent adenylate cyclase system both in normal human thyroid plasma membranes and cultured cells. Iodothyronines at a 30–40 μM concentration did not inhibit the TSH-dependent a-denylate cyclase activity of human thyroid plasma membranes; however at a 1 μM concentration they were able to inhibit the TSH-dependent cAMP accumulation by cultured cells. Preincubation with iodotyrosines and iodothyronine analogues failed to inhibit the TSH-responsive cAMP accumulation in human thyroid cultured cells.This publication has 25 references indexed in Scilit:
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