PERSISTENCE OF DNA DAMAGE DURING DEVELOPMENT OF LIVER ANGIOSARCOMA IN RATS FED DIMETHYLNITROSAMINE

  • 1 January 1979
    • journal article
    • research article
    • Vol. 39  (5) , 1592-1596
Abstract
Chronic feeding of a diet containing dimethylnitrosamine (50 ppm) to rats resulted in liver DNA damage monitored as slow sedimentation of the DNA compared to that of the control in alkaline sucrose gradients. The damage in rat liver DNA could be seen within 2 days after beginning the feeding of diets containing the carcinogen and was progressive with the time of feeding, up to 8 wk. Extended feeding up to 15 or 31 wk did not result in a proportionate increase in the damage to the DNA. The DNA damage observed at 8 wk persisted until 31 wk, at which time liver angiosarcoma was present. Despite the fact that the DNA damage induced by dimethylnitrosamine appears to involve the bulk of the liver DNA, the tumors developed were entirely from vascular endothelium. The implication fo these results in the initiation of carcinogenesis is discussed.

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