The Distribution of Phosphatase in the Spinal Cord of Chick Embryos of One to Eight Days' Incubation

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Abstract
With the histochemical methods of Gomori, both acid and alkaline forms, probably phos-phomonoesterases of classes AI and AII, were demonstrated in spinal cord and other tissues. The alk. reacted more strongly in all stages. Originally uniformly distributed, the alk. became localized in the white matter throughout and in the gray matter and ependyma of the ventral 1/2 of the cord except the motor cells. The acid became largely restricted to these ventral motor groups plus small areas just dorsal and ventral to the lumen. This pattern moved antero-posteriorly during the 4th, 5th and 6th days. Large cells of spinal ganglia had much of both phosphatases. All nucleoli and nuclear membranes were positive for the alkali. Acid was absent from nuclei but was present in a rounded body just outside the nucleus.

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