The Molecular Biology of Rotaviruses. VI. RNA Species-specific Terminal Conservation in Rotaviruses

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Abstract
The use of T1 RNase fingerprinting of terminally labeled genomic double-stranded RNA species from various rotavirus isolates, to analyze the near terminal G-residue positions, has revealed an RNA species-specific fingerprint pattern covering .apprx. 40 nucleotides at the termini. These RNA species-specific terminal fingerprint patterns were found to be conserved in both rotavirus RNA isolated from various animal species, and in isolates from a single animal species where gross divergence of internal RNA sequence for a particular RNA species was evident. This conservation of near terminal G-residue positions suggests that, internal to the short regions of absolute terminal sequence conservation that was previously shown to be present on all rotavirus RNA species, there is a region of conserved sequence which is specific for a particular RNA species.