Tissue-Specific Splicing in Vivo of the β-Tropomyosin Gene: Dependence on an RNA Secondary Structure
- 28 June 1991
- journal article
- other
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 252 (5014) , 1842-1845
- https://doi.org/10.1126/science.2063196
Abstract
The beta-tropomyosin gene in chicken contains two mutually exclusive exons (exons 6A and 6B) which are used by the splicing apparatus in myogenic cells, respectively, before (myoblast stage) and after (myotube stage) differentiation. The myoblast splicing pattern is shown to depend on multiple sequence elements that are located in the upstream intron and in the exon 6B and that exert a negative control over exon 6B splicing. This regulation of splicing is due, at least in part, to a secondary structure of the primary transcript, which limits in vivo the accessibility of exon 6B in myoblasts.Keywords
This publication has 16 references indexed in Scilit:
- RNA Secondary Structure Repression of a Muscle-Specific Exon in HeLa Cell Nuclear ExtractsScience, 1991
- Identification of two distinct intron elements involved in alternative splicing of beta-tropomyosin pre-mRNA.Genes & Development, 1990
- Effects of RNA secondary structure on alternative splicing of Pre-mRNA: Is folding limited to a region behind the transcribing RNA polymerase?Cell, 1988
- Unusual duplex formation in purine rich oligodeoxyribonucleotidesNucleic Acids Research, 1988
- Improved free-energy parameters for predictions of RNA duplex stability.Proceedings of the National Academy of Sciences, 1986
- The role of nucleotide sequences in splice site selection in eukaryotic pre-messenger RNANature, 1986
- Alternative splicing caused by RNA secondary structureCell, 1985
- STRUCTURE OF RIBOSOMAL RNAAnnual Review of Biochemistry, 1984
- Optimal computer folding of large RNA sequences using thermodynamics and auxiliary informationNucleic Acids Research, 1981
- A new technique for the assay of infectivity of human adenovirus 5 DNAVirology, 1973