Gene D of bacteriophage phi X 174: absence of transcriptional and translational regulatory properties

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Abstract
The only evidence that a gene product of .vphi.X174 is involved in the regulation of phage transcription was presented previously for gene D. The D protein had no regulatory effect [in this study]. When the D protein was eliminated by various nonsense mutations, all other phage proteins were synthesized [in Escherichia coli] at rates nearly identical to the wild-type control. There was also no significant difference in the amount of phage-specific RNA synthesis as a proportion of the total RNA synthesis when the D mutants were compared with wild type. No evidence was detected for a polar effect of gene D nonsense mutants on the expression of genes F, G and H. The relative molar rates of synthesis of D, F, G and H [proteins] synthesized during a normal infection were 29, 4.9, 3.3 and 1.0, respectively, with an accuracy of .+-. 20%.