Transformation of sugarcane protoplasts by direct uptake of a selectable chimaeric gene
- 1 July 1987
- journal article
- research article
- Published by Springer Nature in Plant Cell Reports
- Vol. 6 (4) , 297-301
- https://doi.org/10.1007/bf00272003
Abstract
Sugarcane protoplasts were transformed to kanamycin resistance at a frequency of approximately 8 in 107 following PEG-induced uptake of Sma1 linearised pABD1 plasmid. DNA-treated protoplasts were cultured in agarose droplets, and protoplast-derived transformants selected on 80 μg ml−1 kanamycin. Transformed tissues maintained on this level of antibiotic expressed APH(3′)II activity, and contained DNA that hybridised to a probe with the APH(3′)II gene.Keywords
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