Evidence from UV transcription mapping in HeLa cells that heterogeneous nuclear RNA is the messenger RNA precursor.
- 1 October 1977
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 74 (10) , 4520-4523
- https://doi.org/10.1073/pnas.74.10.4520
Abstract
The effects of UV irradiation on the incorporation of [3H]uridine in HeLa (human) cell mRNA, rRNA, heterogeneous nuclear RNA (hnRNA) and early mRNA from adenovirus type 2 have been compared. The UV target size of cell mRNA is at least 3 times larger than the average size of the mRNA itself and larger than the adenovirus-2 early mRNA, which is known to derive from transcription units of about 1.5-5.0 kilobases. The UV target size of hnRNA, in contrast, is about the same as its size determined by sedimentation and overlaps with the target size of mRNA. It is concluded that most mRNA derives from a higher molecular weight hnRNA molecule.This publication has 19 references indexed in Scilit:
- Globin RNA precursor molecules: Biosynthesis and processing in erythroid cellsCell, 1977
- The definition of a large viral transcription unit late in Ad2 infection of HeLa cells: Mapping of nascent RNA molecular labeled in isolated nucleiCell, 1977
- The definition of a large viral transcription unit late in Ad2 infection of HeLa cells: Mapping by effects of ultraviolet irradiationCell, 1977
- The topography and transcription of the adenovirus genomeCell, 1977
- A radiological analysis of the transcription units for heterogeneous nuclear RNA in cultured murine cellsCell, 1976
- hnRNA in HeLa cells: Distribution of transcript sizes estimated from nascent molecule profileCell, 1976
- A precursor of globin messenger RNAJournal of Molecular Biology, 1976
- RNA Synthesis and Processing in Adenovirus-infected CellsCold Spring Harbor Symposia on Quantitative Biology, 1974
- Further evidence on the nuclear origin and transfer to the cytoplasm of polyadenylic acid sequences in mammalian cell RNAJournal of Molecular Biology, 1973
- Amino Acid Metabolism in Mammalian Cell CulturesScience, 1959