Monoclonal Antibodies to an Immunoglobulin Allotype Marker Glm(f)

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Abstract
The properties of a group of mouse monoclonal antibodies (McAbs) specific for the human Glm(f) allotype marker on immunoglobulin G are described. The specificity of all 5 McAbs was anti‐Glm(f) in haemagglutination assays detecting the Glm(f) determinant on 6 ng of purified Glm(f) paraproteins. A high dilution (≥1/104) could be used for the majority of McAbs in this assay. In Elisa the Glm(f) marker could be detected in homozygote Glm(f+z‐) sera at a serum dilution of ≥ 1/104. In Elisa assays the Glm(f) specificity was lost when IgG was bound directly to polystyrene but was restored when IgG was bound via anti‐human IgG to the polystyrene plate. A possible conformational change in IgG to account for this loss of specificity is discussed. It is expected that these McAbs with their high titre and increased sensitivity over conventional Gm antisera will allow more detailed analysis of the Gm marker system.