Carbon Dioxide and Fruiting in Sphaerobolus

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Abstract
In cultures bathed in air deprived of its CO2 fruiting in Sphaerobolus is greatly reduced or fails completely. However, increasing the concentration of CO2 in the air to 1or 5 per cent tends to reduce fruiting as compared with normal air. The stimulatory effect of CO2 in normal air appears to be exerted at the stage of development when sporophore primordia are being produced. The CO2 does not seem to require the immediate presence of light to exert its effect. It has not been found possible to replace the effect of CO2 by substituting Krebs-cycle organic acids. Using 14CO2 it is shown that 14C is incorporated in the mycelium both in light and in darkness.

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