A CONTINUOUS-FLOW CULTURE APPARATUS: THE CYCLONE COLUMN UNIT

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Abstract
An open-type continuous-flow culture apparatus is described, with a working capacity of 500 ml culture. The glass culture vessel, of novel design, permits strict chemostat operation, and has the additional advantages of preventing wall growth, of operating without need for antifoam addition, and of growing filamentous microorganisms without plugging the apparatus. The agitation and mixing produce completely homogenous conditions in the culture; consequently representative sampling and product removal are obtained. Species of anaerobic and aerobic bacteria, yeasts, and micro- and macro-fungi have been grown in the apparatus, in runs which exceeded 4 months" duration. The apparatus is simple to use, reliable in operation, and suitable for wide laboratory use with non-pathogenic organisms.

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