Alkalimetric microassay of cephalosporins.
- 1 January 1978
- journal article
- research article
- Published by Japan Antibiotics Research Association in The Journal of Antibiotics
- Vol. 31 (8) , 776-782
- https://doi.org/10.7164/antibiotics.31.776
Abstract
Alkalimetric pH-stat titrations of cephalosporin C, cephacetril and their deacetyl derivatives using an acetyl esterase and .beta.-lactamase are described. The esterase was used to assay highly purified samples of cephalosporin C and cephacetril and also to prepare analytically defined solutions of the deacetyl cephalosporins. Lactamase-catalyzed hydrolysis of the parent compounds generated exactly 2 equivalents of acid/mol; that of the deacetyl derivatives exactly 1 equivalent.This publication has 4 references indexed in Scilit:
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- High performance liquid chromatography of natural products. I. Separation of cephalosporin C derivatives and cephalosporin antibiotics; isolation of cephalosporin C from fermentation broth.The Journal of Antibiotics, 1976
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