Adherence properties ofPseudomonaspili to epithelial cells of the human cornea
- 1 January 1982
- journal article
- research article
- Published by Taylor & Francis in Current Eye Research
- Vol. 2 (5) , 289-293
- https://doi.org/10.3109/02713688209000772
Abstract
In vitro adherence of Pseudomonas fluorescens organisms to the human cornea is correlated with bacterial pili. The role of pili in the attachment to human corneal epithelial cells was studied in an in vitro adherence assay system. A homogeneous, purified pilin preparation showed a molecular weight of 16,500 on SDS-polyacrylamide gels. Within 5 minutes incubation, 5.5 pg of pilin adhere to 15,000 epithelial cells. When epithelial cells were preincubated with purified pilin, a subsequent decrease in adherence of labeled pilin was noted. It appears that pili mediate adherence of Pseudomonas organism to human cornea.This publication has 18 references indexed in Scilit:
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