Interaction of a synaptobrevin (VAMP)‐syntaxin complex with presynaptic calcium channels

7 March 1995

journal article
Published by Wiley in FEBS Letters

Vol. 361 (1) , 101-105
https://doi.org/10.1016/0014-5793(95)00156-4

Abstract

Nerve terminal protein complexes implicated in exocytosis were examined by immuno‐isolation from rat brain synaptosomes. Immunoprecipitation with anti‐syntaxin or anti‐VAMP antibodies revealed a syntaxin‐SNAP25‐VAMP‐synaptotagmin complex. Anti‐VAMP antibodies also trapped a distinct VAMP‐synaptophysin complex. A similar fraction (about 70%) of N‐type calcium channels ([¹²⁵I]ω conotoxin GVIA receptors), was immunoprecipitated by either anti‐syntaxin or anti‐VAMP antibodies, but not by anti‐synaptophysin antibodies (< 4%). The majority of N‐ but not L‐type calcium channels ([³H]PN200‐110 receptors), appear to be associated with a synaptic vesicle prefusion complex.

Keywords

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