Characterization of Microsomal Subfractions from Porcine Endometrium Cells

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Abstract
Crude microsomes from porcine endometrium and 3 subfractions obtained by a modification of Rothschild''s technique were characterized by RNA/protein ratio, marker enzyme activities and morphological appearance. The microsomes were devoid of glucose-6-phosphatase activity. They contained .apprx. 10% of arylesterase-, .apprx. 30% of both NADPH-cytochrome reductase- and UDP-galactose-N-acetyl-glucosamine .beta.-D-galactosyltransferase- and .apprx. 60% of 5''-nucleotidase activities present in the homogenates. Subfraction I (smooth membranes) had twice the galactosyltransferase activity of Subfraction II (smooth and rough membranes + free ribosomes); both subfractions were rich in 5''-nucleotidase and cytochrome reductase activities. Subfraction III (rough membranes) had very low marker activities but exhibited the highest RNA/protein ratio, which was lowest in I.