The human β-globin gene contains a downstream developmental specific enhancer
Open Access
- 24 July 1987
- journal article
- research article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 15 (14) , 5739-5747
- https://doi.org/10.1093/nar/15.14.5739
Abstract
The human β-globin gene is part of a multigene fsmily and is expressed specifically in adult human erythroid tissue (for review, 1). When the human β-globin is introduced into fertilized mouse eggs, it is first activated in foetal liver and remains expressed in adult erythroid tissues (2,3,4). It therefore mimicks the pattern of expression of its murine counterpart. It has previously been shown in tissue culture (5) and transgenic mice (4) that sequences downstream from the β-globin promoter are involved in this regulation. We now show that at least part of these sequences are located 0.5–1.2kb downstream from the polyA addition site and constitute a transcriptional enhancer element that is erythroid and developmental specific.Keywords
This publication has 25 references indexed in Scilit:
- Regulated expression of human Aγ-, β-, and hybrid γβ-globin genes in transgenic mice: Manipulation of the developmental expression patternsCell, 1986
- Regulated gene expression in transfected primary chicken erythrocytes.Proceedings of the National Academy of Sciences, 1986
- The human β-interferon gene enhancer is under negative controlCell, 1986
- An embryonic pattern of expression of a human fetal globin gene in transgenic miceNature, 1986
- Detailed analysis of the mouse H-2Kb promoter: Enhancer-like sequences and their role in the regulation of class I gene expressionCell, 1986
- Cell-Specific Expression of the Rat Insulin Gene: Evidence for Role of Two Distinct 5′ Flanking ElementsScience, 1985
- Mechanisms that Regulate Immunoglobulin Gene ExpressionAnnual Review of Immunology, 1985
- Embryonic erythroid differentiation in the human leukemic cell line K562.Proceedings of the National Academy of Sciences, 1981
- Hybridization of a Myeloid Leukemia-Derived Human Cell Line (K562) With a Human Burkitt's Lymphoma Line (P3HR-1)2JNCI Journal of the National Cancer Institute, 1980
- The sequence of the chromosomal mouse β-globin major gene: Homologies in capping, splicing and poly(A) sitesCell, 1978