FURTHER OBSERVATIONS ON GROWTH OF SINGLE CELLS OF COCCOID BLUE‐GREEN ALGAE

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Abstract
SUMMARY: Initiation of growth (lag) and subsequent growth to visible colonies was examined for single cells of several coccoid blue‐nreen algae under controlled incuba‐tion conditions. At 30 or 39 C, with tungsten or fluorescent illumination, organisms such as Agmenel‐lum quadruplicatum, strains PR‐6 and strain BG‐J, showed no evidence of lag associated with initiation of growth. The final colony count was within 20% of the expected number derived from cell counts and serial dilutions. For Anacystis nidulnns, Tx 20, a new medium C, 10 with EDTA as chelator was developed. In this medium groiuth of single cells at 39 C was excellent with quantitative recovery and no evidence of lag. At 30 C, however, Tx 20 showed anomalous behavior, lag and nonquantitative cell recovery. This behavior at 30 C is not yet understood.

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