The Serum Bactericidal Reaction. III. Antibody and Complement Requirements for Killing a Rough Escherichia Coli

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Abstract
Summary: The serum bactericidal reaction was studied using the rough E. coli 200P and a system of serum reagents made selectively deficient in various complement components. In replacement experiments functionally pure individual components were added and requirements for C1, C4 and C2, and C9 as well as antibody were shown. Cellular intermediates BAC1 and BAC142 were demonstrated. The results suggest that the entire complement system is required to kill bacteria. Note Added in Proof. Inoue et al. (Inoue, K., Yonemasu, K., Takamizawa, A. and Amano, T. Biken J., 11: 203, 1968) have recently reported that BAC142 cells, C3, 5, 6, 7, 8, 9 and lysozyme were required for immune bacteriolysis of a rough strain of E. coli. The complement components used were purified from guinea pig serum. Results were based on observations of spheroplast formation: with the complete system about 25% of the rod-shaped bacteria were converted to spheroplasts.

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