‘Cap 90’, a 90‐kDa Ca2+‐dependent F‐actin‐capping protein from vertebrate brain
- 14 November 1983
- journal article
- Published by Wiley in FEBS Letters
- Vol. 163 (2) , 225-229
- https://doi.org/10.1016/0014-5793(83)80824-2
Abstract
A Ca2+‐dependent actin filament‐capping protein of 90 kDa was purified from bovine brain using a new and rapid isolation procedure. This basically includes affinity purification on DNase‐I agarose. The protein caps the fast‐growing end of actin filaments but has no fragmenting or severing activity. Using Triton X‐100‐extracted cytoskeletons, capping and severing activities of actin‐binding proteins become clearly distinguishable from each other.Keywords
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