Relation between Structure and Function in Some Partially Synthetic Ribonucleases S′
Open Access
- 3 March 1975
- journal article
- Published by Wiley in European Journal of Biochemistry
- Vol. 52 (1) , 65-76
- https://doi.org/10.1111/j.1432-1033.1975.tb03973.x
Abstract
Some analogues have been prepared of S‐peptide, the peptide obtained together with S‐protein from subtilisin‐modified beef pancreatic RNase A. The syntheses are described of [Orn10, Asn14]‐S‐peptide and 1ɛ, 7ɛ,10δ‐triguanidino‐[Orn10, Asn14]‐S‐peptide. The S‐peptide analogues are able to activate S‐protein at the level of the parent [Orn10]‐S‐peptide and 1ɛ,7ɛ‐diguanidino‐S‐peptide respectively, although at high peptide‐to‐protein molar ratios. After their recombination with S‐protein the buried character of Tyr‐25 was restored, as judged from difference absorption and circular dichroism spectra in the near‐ultraviolet region. These findings indicate that the asparaginyl residue is a possible naturally occurring substituent in the RNase A sequences whose state of amidation in position 14 has not yet been defined.Keywords
This publication has 36 references indexed in Scilit:
- Studies on cytochrome c. Part II. Synthesis of the protected heptapeptide (sequence 17–23) of Baker's yeast iso‐1‐cytochrome cBiopolymers, 1973
- Preparation and crystallization of 41-dinitrophenyl ribonuclease SBiochemistry, 1971
- Thermodynamics of the binding of S-peptide to S-protein to form ribonuclease S'Biochemistry, 1971
- Polypeptides. XLIV. Potent synthetic S-peptide antagonistsJournal of the American Chemical Society, 1970
- Analysis of the vibrational structure in the near-ultraviolet circular dichroism and absorption spectra of tyrosine derivatives and ribonuclease-A at 77.deg.KJournal of the American Chemical Society, 1970
- Conformational‐transitions of bovine pancreatic ribonuclease S‐peptideFEBS Letters, 1968
- Studies on Polypeptides. XXXIV. Enzymic Properties of Partially Synthetic De(16-20)- and De(15-20)-ribonucleases S'1-3Journal of the American Chemical Society, 1966
- Studies on Polypeptides. XXXIII. Enzymic Properties of Partially Synthetic Ribonucleases1-4Journal of the American Chemical Society, 1965
- Structural Studies of Ribonuclease. V. Reversible Change of Configuration1-3Journal of the American Chemical Society, 1961
- Phenolic Hydroxyl Ionization in Proteins. II. Ribonuclease1aJournal of the American Chemical Society, 1955