RESPONSE OF IRON-DEFICIENT BARLEY PLANTS TO MANGANESE IN NUTRIENT SOLUTION

Abstract
Barley (Hordeum vulgare L. cv. Minorimugi) plants were cultured in iron (Fe)-deficient nutrient solutions at three levels (0.25, 0.025, and 0.0025 μM) of manganese (Mn). Iron-deficient plants with high Mn (0.25 μM) had brown spots on older leaves and stems characteristic of Mn toxicity. In the nutrient solution at 0.0025 μM Mn, the younger leaves had typical symptoms of Mn deficiency. Iron-deficient plants with 0.025 μM Mn had shoot Mn concentration statistically similar to Fe-sufficient plants with 0.25 μM Mn. Tissue Fe and Mn concentrations were reciprocally related in shoots and roots of plants grown in 0.25 μM Mn with and without Fe. The Mn concentration of Fe-deficient plants decreased with decreasing Mn concentrations in the nutrient solution. In contrast, the Mn levels in the nutrient solution did not significantly affect the Fe concentrations of Fe-deficient plants with identical Fe deficiency symptoms. The highest level of phytosiderophore (PS) released from roots of Fe-deficient plants occurred ...
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