Genetic structure and transcriptional analysis of the arginine deiminase (ADI) cluster inLactococcus lactisMG1363
- 1 July 2006
- journal article
- Published by Canadian Science Publishing in Canadian Journal of Microbiology
- Vol. 52 (7) , 617-622
- https://doi.org/10.1139/w06-009
Abstract
In a recent proteomic analysis, we showed the overproduction of the ArcA and ArcB proteins in Lactococcus lactis MG1363 at low pH. The corresponding genes belong to the arcABD1C1C2TD2 cluster that encodes components of the arginine deiminase pathway. In this study, we characterized this cluster at the genetic level. Northern blot experiments showed the expression of at least seven transcripts, all induced by acidity. Transcript analysis using 5'RACE PCR (rapid amplification of cDNA ends polymerase chain reaction) in the arcB-arcD1 intergenic region. In silico analysis identified nine stem-loop structures, all located in intergenic regions. Collectively, these data suggest a role for RNA processing and (or) premature termination in the differential expression of genes within the arcABD1C1C2TD2 cluster.Keywords
This publication has 22 references indexed in Scilit:
- Proteomic characterization of the acid tolerance response inLactococcus lactis MG1363Proteomics, 2005
- Characterization of the Arginine Deiminase Operon of Streptococcus rattus FA-1Applied and Environmental Microbiology, 2004
- ArticleLe Lait, 2003
- Surviving the Acid Test: Responses of Gram-Positive Bacteria to Low pHMicrobiology and Molecular Biology Reviews, 2003
- RNA Processing and Degradation inBacillus subtilisMicrobiology and Molecular Biology Reviews, 2003
- Gene Structure, Organization, Expression, and Potential Regulatory Mechanisms of Arginine Catabolism inEnterococcus faecalisJournal of Bacteriology, 2002
- Isolation and Molecular Analysis of the Gene Cluster for the Arginine Deiminase System from Streptococcus gordonii DL1Applied and Environmental Microbiology, 2002
- The Complete Genome Sequence of the Lactic Acid Bacterium Lactococcus lactis ssp. lactis IL1403Genome Research, 2001
- Processing of the Pseudomonas arcDABC mRNA requires functional RNase E in Eschirichia coliGene, 1993
- RNA processing modulates the expression of the arcDABC operon in Pseudomonas aeruginosaJournal of Molecular Biology, 1992