Analysis of murine interferon‐γ binding to its receptor on intact cells and solubilized membranes Identification of an 80 kDa receptor

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Abstract
The receptor for murine‐interferon‐γ (Mu‐IFN‐γ) has been characterized for its molecular size and equilibrium binding constant on a thymoma cell line, EL‐4. Binding of 125I‐IFN‐γ to intact cells and their solubilized membranes has shown a single class of receptor with K d values of 1.9 × 10−9 M and 1.3 × 10−8 M, respectively. It was shown that solubilization of the Mu‐IFN‐γ receptor with a Zwitterionic detergent (Chaps) preserves its binding activity. A direct comparison of the molecular mass of the Mu‐IFN‐γ receptor on intact cells versus detergent‐solubilized membranes was performed using a radiolabeled photoactivated crosslinking reagent and direct hybridization with 125I‐labeled IFN‐γ on Western blots of solubilized receptor. The results indicate that both types of receptors have an identical molecular mass of ∼80 kDa.