Effect of some cultural factors on T1 broth vaccine for contagious bovine pleuropneumonia
- 1 September 1983
- journal article
- research article
- Published by Springer Nature in Tropical Animal Health and Production
- Vol. 15 (3) , 144-148
- https://doi.org/10.1007/bf02239922
Abstract
The effect of some aspects of buffering capacity, glucose concentration, length of incubation and extent of exposure to air during the preparation of liveMycoplasma mycoides var.mycoides strain T1 broth culture vaccine was investigated. A consistently good vaccine was obtained using 0.07M phosphate buffered medium containing 0.1% glucose and incubating in stoppered bottles. The shelf-life of this vaccine stored at 4°C was up to 12 weeks. The effect of other storage temperatures on the viability of the vaccine is given. Les effets de certains aspects de l'action tampon, la concentration de glucose, la durée d'incubation et d'exposition à l'air pendant la préparation du vaccin de culture vivant contreMycoplasma mycoides var.mycoides (souche T1) ont été observés. Un bon vaccin a été obtenu de façon constante en utilisant un milieu tamponné au phosphate à 0,07M contenant 0,1 p. 100 de glucose et étant mis à incuber dans des flacons bouchés. La durée de conservation de ce vaccin stocké 4°C s'étend jusqu'à douze semaines. Les effets d'autres températures sur la viabilité du vaccin sont décrits. Se investigaron los efectos de algunos aspectos tales cómo, capacidad tampón, concentración de glucosa, tiempo de incubación y extensión de la exposición al aire, durante la preparación de la caldo-vacuna deMicoplasma mycoides var. mycoides cepa T1. Consistentemente se obtuvo una buena vacuna, utilizando un medio tampón fosfatado 0.07M con 0.1% de glucosa e incubación en botellas cerradas. La vida de la caldo-vacuna at 4°C fué de de 12 semanas; se habla tambien de la vida promedio de la mísma a diferentes temperaturas.Keywords
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