Flow cytometry analysis of α1‐adrenoceptor subtypes
- 20 May 1996
- journal article
- Published by Wiley in FEBS Letters
- Vol. 386 (2-3) , 141-148
- https://doi.org/10.1016/0014-5793(96)00388-2
Abstract
To characterize the α1-adrenoceptor subtypes, we developed a flow cytometry method using the fluorescent ligand BODIPY-FL prazosin and the anti-peptide antibody against the α1b-adrenoceptor amino terminus (designated 1B-N1-C) as probes. Three α1-adrenoceptors (α1a, β1b and α1d) expresed in CHO cells were detected by BODIPY-FL prazosin; however, only α1b-adrenoceptor subtype was detected by the anti-peptide antibody 1B-N1-C. Furthermore, the flow cytometry analysis with 1B-N1-C specifically identified α1b-adrenoceptor in native cells of hamster DDT1-MF2 cells, rat hepatocytes and1 cardiomyocytesKeywords
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