Inhibitory Effects of T cells on In Vitro Granulopoiesis, Erythropoiesis, and Immunoglobulin Production in Patients with Aplastic Anaemia

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Abstract
Coculture with mononuclear cells from aplastic anaemia (AA) patients and normal bone marrow cells produced different responses among 10 patients studied. In 4 cases, CFU‐E growth was inhibited while in another 2 cases CFU‐C growth was inhibited. To determine the suppressive effects of mononuclear cells from 6 AA patients, mononuclear cells were separated into T cells, B cells, and monocytes. It was found that T cells from 6 AA patients exhibited suppressive effects on normal CFU‐C or CFU‐E growth, whereas T cells from normal subjects enhanced the colony formation of CFU‐C and CFU‐E. On the other hand, monocytes from 6 A A patients and normal subjects strongly suppressed normal CFU‐C and CFU‐E growth, although no significant difference was observed in both groups. The immune system in 10 AA patients was evaluated. 3 AA patients (Cases No. 1, 6, 8) exhibited an increase in Tr cells, impairment of PHA responsiveness, and low level of immunoglobulin. T cells from these patients showed a significant suppressive effect on PWM‐induced differentiation of allogenic normal B cells. Thus, T cells from 3 out of 10 AA patients exhibited suppressor activities on both haematopoietic cell differentiation and B cell differentiation into plasma cells in vitro.