Alleviation of type I restriction in adenine methylase (dam) mutants of Escherichia coli
- 1 October 1988
- journal article
- research article
- Published by Springer Nature in Molecular Genetics and Genomics
- Vol. 214 (2) , 313-316
- https://doi.org/10.1007/bf00337727
Abstract
The host-controlled EcoK-restriction of unmodified phage λ.O is alleviated in dam mutants of Escherichia coli by 100- to 300-fold. In addition, the EcoK modification activity is substantially decreased in dam - strains. We show that type I restriction (EcoB, EcoD and EcoK) is detectably alleviated in dam mutants. However, no relief of EcoRI restriction (Type II) occurs in dam - strains and only a slight effect of dam mutation on EcoP1 restriction (Type III) is observed. We interpret the alleviation of the type I restriction in dam - strains to be a consequence of induction of the function which interferes with type I restriction systems.Keywords
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