Preparation of and Glucose Absorption from Intestinal Loops in Conscious Swine

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Abstract
Techniques are described for surgical preparation of isolated loops of small intestine in swine. Methods for training and post-surgical care of animals are outlined, and apparatus for perfusion of the intestinal loops are described. The viability of the loops was established by demonstration of active glucose transport. The rate of glucose absorption from isolated loops of duodenum and ileum was dependent upon the initial glucose concentration, and this relationship followed Michaelis-Menten kinetics. Km and Vmax coefficients are reported. Saturation and phlorizin inhibition of glucose transport were both demonstrated. Copyright © 1977. American Society of Animal Science . Copyright 1977 by American Society of Animal Science.

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