Alteration in radiation-induced change in K+ permeability of mouse fibroblast LM cells by modification of their membranes with unsaturated fatty acids.

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Abstract
The membrane lipid of murine fibroblast LM cells, which can grow in serum-free medium, was modified by adding an unsaturated fatty acid (palmitoleate, oleate, linoleate or linolenate) to the medium. After irradiation with 60Co γ-rays at 0°C or 25°C, these cells lost their intracellular K+ with time, and the degree of loss varied depending on the modification of their membranes: palmitoleate-supplemented membranes were most resistant to radiation, whereas linolenate-supplemented ones were least resistant at 4°C and 25°C. No membrane constituents other than lipids seemed to be appreciably modified, judging from the indirect evidence that the rates of 42K incorporation into linolenate-supplemented and -unsupplemented cells at 37°C were the same. Thus, these differences in the damage indicate that membrane lipids take part in the radiation-induced change in K+ permeability of LM cells.

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