Abstract
Addition to de-aerated malt wort of ergosterol dispersed in the non-lipid detergent Tergitol allows satisfactory growth of oxygen-requiring cells of brewing yeast. Any nutritional requirement for unsaturated fatty acid for viability and growth of such cells (as distinct from effect on metabolite balance, e.g. of esters) can therefore be supplied by the normal constituents of a typical malt wort.