Detection of Viral Proteins in Cytopathic Structures in Cowpea Protoplasts Infected with Cowpea Mosaic Virus
- 1 March 1988
- journal article
- research article
- Published by Microbiology Society in Journal of General Virology
- Vol. 69 (3) , 751-755
- https://doi.org/10.1099/0022-1317-69-3-751
Abstract
Infection of cowpea (Vigna unguiculata) cells or protoplasts with cowpea mosaic virus is accompanied by the appearance of characteristic cytopathic structures. A major constituent of these cytopathic structures, represented in thin sections by electron-dense material, was shown to contain viral non-structural proteins by its reaction with antisera to non-structural proteins and their detection with colloidal gold-labelled Protein A. With this same technique virus particles were found to accumulate mainly in the cytoplasm.This publication has 6 references indexed in Scilit:
- The Genome-linked Protein of Cowpea Mosaic Virus Is Bound to the 5' Terminus of Virus RNA by a Phosphodiester Linkage to SerineJournal of General Virology, 1987
- Detection of a Novel Protein Encoded by the Bottom-Component RNA of Cowpea Mosaic Virus, Using Antibodies Raised against a Synthetic PeptideJournal of Virology, 1987
- Purification of cowpea mosaic virus RNA replication complex: Identification of a virus-encoded 110,000-dalton polypeptide responsible for RNA chain elongationProceedings of the National Academy of Sciences, 1984
- Localization by Immunogold Cytochemistry of Viral Antigen in Sections of Plant Cells Infected with Red Clover Mottle VirusJournal of General Virology, 1983
- Limits to the Independence of Bottom Component RNA of Cowpea Mosaic VirusJournal of General Virology, 1982
- Expression of Bottom Component RNA of Cowpea Mosaic Virus in Cowpea ProtoplastsJournal of Virology, 1980