Electroporation Stimulates Plant Regeneration from Protoplasts of the Woody Medicinal SpeciesSolarium dulcamaraL.

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Abstract
Exposure of cell suspension protoplasts of the woody medicinal plant Solatium dulcamara L. to voltages of 250 to 1250 V cm−1 for three successive pulses, each of 10–50 us duration, stimulated growth of protoplast-derived tissues. Such tissues exhibited increased morphogenesis and required a shorter period in culture to exhibit this effect than tissues from untreated protoplasts. Regenerated shoots also rooted more readily and developed more prolific root systems than shoots from untreated protoplasts. These observations have important implications for plant genetic manipulation and may have application in the recovery and rooting of shoots from tissues of woody species, normally considered recalcitrant in culture.