Spectrophotometric Assay of Certain Aminoglycosoide Antibiotic Using Chloranil

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Abstract
Chloranil (2,3,5,6-teterachloro-p-benzoquinone) reacts with certain aminoglycosoide antibiotics in borate buffer at pH 9.0 to give charge-transfer complexes. The complexes possese an absorption maxmum at about 350 nm with molar absorptivities of 3.78 × 105, 4.69 × 105, 5.0 × 105 and 7.08 × 105 for kanamycin, neomycin B, amikacin and tobramycin reepectively, These high intensity of the chargetransfor band and confirmity to beer's law enabled assay of these antibiotics with appreciable sensitivity and good precision. Aminoglycosoide antibiotics have a broad spectrum of activity against both gram-negative and gram-positive bacteria. The official compendia1,2 recommend the microbiological assay for their evalution. The high experimental errors in microbiological estimation heae been reported3. Numerous spectrophotometric, fluorometric and chromatographic techniques for their quantitation have been thoroughly reviewed4,5. Substituted quinones such as 2,5 dichloroquinone6, 7,7,8,8-tetracyanoqulnone dimethane7, flouranil8 are used as TT- acceptors with various electron donors to form charge-transfer(CT) complexes and radical ions9,10. Some aromatic amines11,12 and aminoacids have been determined with chloranil13–15. The present work describes the utility of chloranil for the spectrophotometric determination of kanamycin sulphate, amikacin sulphate tobramycin aulphate, and neorycin B sulphate (framycetine sulphate) either in bulk or in their reepective doaage forms.