Heterologous in vitro transcription from two archaebacterial promoters
- 1 July 1991
- journal article
- Published by Wiley in FEBS Letters
- Vol. 285 (2) , 257-259
- https://doi.org/10.1016/0014-5793(91)80811-g
Abstract
A cell-free extract of Sulfolobus shibatae is able to specifically initiate transcription in vitro at the promoter of the plasmid-encoded gene for the major gas vesicle protein of Halobacterium halobium and at the promoter for the transcript T4 of the temperate H. halobium phage ΦH. The corresponding promoter from the virulent phage mutant ΦHL1 yields enhanced transcription in the heterologous system, in agreement with strongly increased in vivo expressionKeywords
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